Anopheles maculipennis Complex in The Netherlands: First Record of Anopheles daciae (Diptera: Culicidae)

Despite their past importance as vectors of indigenous malaria, the species composition and spatial distribution members Anopheles maculipennis complex have been studied to a limited extent in Netherlands. Therefore, this investigation focuses on Netherlands, including daciae, which has recently found countries bordering In framework national mosquito surveillance between 2010 2021, total 541 specimens An. s.l. were analyzed from 161 locations covering entire territory. addition, 89 overwintering sites during winter 2020/2021. All individual mosquitoes identified species-level using Sanger sequencing ribosomal internal transcribed spacer 2. To characterize habitat land cover use data was extracted 1 km buffer area around each finding location. For populations collected summers most frequent messeae, present 88.19% locations, followed by s.s. (11.80%), atroparvus (3.72%) daciae (3.72%). southern inland areas country. Furthermore, messeae occurred sympatry at sites. This study provides relevant information occurrence contributing better estimation risk mosquito-borne disease